dCas9 can be fused with a gene repressor protein, the KRAB protein, to form a complex CRISPRi (CRISPR interference) that inhibits gene expression. dCas9-KRAB targeting to the promoter region causes steric hindrance that halts transcript elongation by RNA polymerase, resulting in the target gene being repressed. CRISPR-based gene interference provides an efficient and specific genome-targeting platform for transcriptional control without altering the target DNA sequence.

CRISPR/Cas9 Platform^CB provides a complete and powerful solution for CRISPR gene interference helping you to specifically suppress the expression of any gene. Our custom CRISPRi sgRNA vectors can be used on a variety of cells, including easy-to-transfect and difficult-to-transfect cells, covering the entire genome of humans, mice, and rats. Just send us the gene ID you are interested in, we will design, clone and provide this powerful technology for you.

CRISPRi sgRNA vector services include

• Consult our technicians and design your CRISPR-based gene interference protocol
• CRISPRi target site selection
• Design and clone multiple sgRNAs based on target genomic loci
• Virus vector packaging

➢ We offer a variety of sgRNA vector designs, including sgRNA only, sgRNA-dCas9-KRA
➢ We offer a variety of viral vectors (lentivirus, adenovirus, AVV)
➢ We offer TET-inducible or stable expression.